Featured Finding Figure
In the primate neocortex, little is know about the possible associations between functional subclasses of GABA neurons, their morphological properties and calcium-binding protein (CaBP) content. We used whole cell current clamp recordings, combined with intracellular labeling and fluorescence immunohistochemistry to determine these relationships for interneurons in layers 2-3 of monkey prefrontal cortex. As shown in Panel A, physiologically-characterized biocytin-injected interneurons (BC) were found to be positive for parvalbumin (PV), calretinin (CR) or calbindin (CB). Co-localization of more than one CaBP was not observed in any of the interneurons examined. Interneurons with different CaBPs formed distinct populations with specific physiological membrane properties and morphological features. PV-positive cells had the physiological properties characteristic of fast-spiking interneurons and the morphology of basket or chandelier neurons, whereas most CR-containing cells had the physiological properties ascribed to non-fast-spiking cells and a vertically-oriented axonal morphology, similar to that of double bouquet cells. CB-positive interneurons also had non-fast-spiking properties and included cells with double bouquet morphology or with a characteristic dense web of axonal collaterals in layer 1. Cluster analysis, summarized in Panel B, of multiple electrophysiological properties suggested the existence of at least two distinct classifications of interneurons. The first group contained mainly PV-positive fast-spiking cells and the second group consisted exclusively of CR- and CB-positive non-fast-spiking interneurons. These findings may help to illuminate the functional roles of different groups of interneurons in primate prefrontal circuitry.
Zaitsev AV, Gonzalez-Burgos G, Povysheva NV, Kroner S, Lewis DA, Krimer LS. Localization of calcium-binding proteins in physiologically and morphologically characterized interneurons of monkey dorsolateral prefrontal cortex. Cerebral Cortex 15: 1178-1186, 2005.

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David A. Lewis, M.D. | Department of Psychiatry | University of Pittsburgh
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